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5. | | VARGAS, M. A. T.; CUNHA, M. H. da. Legume inoculation in cerrado soils. In: INTERNATIONAL CONGRESS ON NITROGEN FIXATION, 12., 1999, Foz do Iguacu. Nitrogen fixation: from molecules to crop productivity: proceedings. Dordrechet: Kluwer, 2000. p. 587-588. (Current Plant Science and Biotechnology in Agriculture, 38). Editado por Fabio O. Pedrosa, Mariangela Hungria, Geoffrey Yates, William E. Newton. Biblioteca(s): Embrapa Soja. |
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6. | | MERCANTE, F. M.; MENDES, I. de C.; REIS JUNIOR, F. B. dos; CUNHA, M. H. da; REIS JUNIOR, F. B. dos; CUNHA, M. H. da. SPD favorece a fixação biológica de nitrogênio na soja. A Granja, Porto Alegre, ano 68, n. 757, p. 77-79, jan. 2012. Biblioteca(s): Embrapa Soja. |
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14. | | BORTOLAN, S.; BARCELLOS, F. G.; MARCELINO, F. C.; CUNHA, M. H. da. Expressão dos genes nodC, nodW e nopP em Bradyrhizobium japonicum estirpe CPAC 15 avaliada por RT-qPCR. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 44, n. 11, p. 1491-1498, nov. 2009. Biblioteca(s): Embrapa Agricultura Digital. |
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15. | | ZILLI, J. E.; GIANLUPPI, V.; CAMPO, R. J.; CUNHA, M. H. da. Método para inoculação de soja por pulverização em cobertura. In: REUNIÃO DA REDE DE LABORATÓRIOS PARA RECOMENDAÇÃO, PADRONIZAÇÃO E DIFUSÃO DE TECNOLOGIA DE INOCULANTES MICROBIOLÓGICOS DE INTERESSE AGRÍCOLA, 14., 2008, Bonito. Anais... Dourados: Embrapa Agropecuária Oeste, 2010. p. 44-45. RELARE. Organizado por Fábio M. Mercante, Oscar F. de Lima Filho, Suelma P. da S. Bonatto. Biblioteca(s): Embrapa Soja. |
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20. | | CARDOSO, J. D.; GOMES, D. F.; MATOS, M. A. de; ANDRADE, D. S.; CUNHA, M. H. da. Caracterização inter e intra-específica de estirpes elite de rizóbio. In: CONGRESSO BRASILEIRO DE MICROBIOLOGIA, 25., 2009, Porto de Galinhas. Anais... [São Paulo]: Sociedade Brasileira de Microbiologia, 2009. Seção Resumos, ref. 2364-1. 1 CD-ROM. Biblioteca(s): Embrapa Soja. |
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Registros recuperados : 29 | |
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| Acesso ao texto completo restrito à biblioteca da Embrapa Soja. Para informações adicionais entre em contato com valeria.cardoso@embrapa.br. |
Registro Completo
Biblioteca(s): |
Embrapa Soja. |
Data corrente: |
21/12/2011 |
Data da última atualização: |
17/04/2018 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
B - 2 |
Autoria: |
TORRES, A. R.; KASCHUK, G.; SARIDAKIS, G. P.; CUNHA, M. H. da. |
Afiliação: |
ADALGISA RIBEIRO TORRES; GLACIELA KASCHUK, Universidade Paranaense; GEORGE P. SARIDAKIS; MARIANGELA HUNGRIA DA CUNHA, CNPSO. |
Título: |
Genetic variability in Bradyrhizobium japonicum strains nodulating soybean [Glycine max (L.) Merrill]. |
Ano de publicação: |
2011 |
Fonte/Imprenta: |
World Journal of Microbiology and Biotechnology, Dec. 2011. |
DOI: |
10.1007/s11274-011-0964-3 |
Idioma: |
Inglês |
Conteúdo: |
Brazil has succeeded in sustaining production of soybean [Glycine max (L.) Merrill] by relying mainly on symbiotic N2 fixation, thanks to the selection and use in inoculants of very effective strains of Bradyrhizobium japonicum and Bradyrhizobium elkanii. It is desirable that rhizobial strains used in inoculants have stable genetic and physiological traits, but experience confirms that rhizobial strains nodulating soybean often lose competitiveness in the field. In this study, soybean cultivar BR 16 was singleinoculated with four B. japonicum strains (CIAT 88, CIAT 89, CIAT 104 and CIAT 105) under aseptic conditions. Forty colonies were isolated from nodules produced by each strain. The progenitor strains, the isolates and four other commercially recommended strains were applied separately to the same cultivar under controlled greenhouse conditions. We observed significant variability in nodulation, shoot dry weight, shoot total N, nodule efficiency (total N mass over nodule mass) and BOX-PCR fingerprinting profiles between variant and progenitor strains. Some variant strains resulted in significantly larger responses in terms of shoot total N, dry weight and nodule efficiency, when compared to their progenitor strain. These results highlight the need for intermittent evaluation of stock bacterial cultures to guarantee effective symbiosis after inoculation. Most importantly, it indicates that it is possible to improve symbiotic effectiveness by screening rhizobial strains for higher N2 fixation capacity within the natural variability that can be found within each progenitor strain. MenosBrazil has succeeded in sustaining production of soybean [Glycine max (L.) Merrill] by relying mainly on symbiotic N2 fixation, thanks to the selection and use in inoculants of very effective strains of Bradyrhizobium japonicum and Bradyrhizobium elkanii. It is desirable that rhizobial strains used in inoculants have stable genetic and physiological traits, but experience confirms that rhizobial strains nodulating soybean often lose competitiveness in the field. In this study, soybean cultivar BR 16 was singleinoculated with four B. japonicum strains (CIAT 88, CIAT 89, CIAT 104 and CIAT 105) under aseptic conditions. Forty colonies were isolated from nodules produced by each strain. The progenitor strains, the isolates and four other commercially recommended strains were applied separately to the same cultivar under controlled greenhouse conditions. We observed significant variability in nodulation, shoot dry weight, shoot total N, nodule efficiency (total N mass over nodule mass) and BOX-PCR fingerprinting profiles between variant and progenitor strains. Some variant strains resulted in significantly larger responses in terms of shoot total N, dry weight and nodule efficiency, when compared to their progenitor strain. These results highlight the need for intermittent evaluation of stock bacterial cultures to guarantee effective symbiosis after inoculation. Most importantly, it indicates that it is possible to improve symbiotic effectiveness by screening rhizobial strains fo... Mostrar Tudo |
Thesagro: |
Fixação de nitrogênio; Soja. |
Thesaurus NAL: |
Nitrogen fixation; Soybeans. |
Categoria do assunto: |
S Ciências Biológicas |
Marc: |
LEADER 02254naa a2200217 a 4500 001 1910598 005 2018-04-17 008 2011 bl uuuu u00u1 u #d 024 7 $a10.1007/s11274-011-0964-3$2DOI 100 1 $aTORRES, A. R. 245 $aGenetic variability in Bradyrhizobium japonicum strains nodulating soybean [Glycine max (L.) Merrill]. 260 $c2011 520 $aBrazil has succeeded in sustaining production of soybean [Glycine max (L.) Merrill] by relying mainly on symbiotic N2 fixation, thanks to the selection and use in inoculants of very effective strains of Bradyrhizobium japonicum and Bradyrhizobium elkanii. It is desirable that rhizobial strains used in inoculants have stable genetic and physiological traits, but experience confirms that rhizobial strains nodulating soybean often lose competitiveness in the field. In this study, soybean cultivar BR 16 was singleinoculated with four B. japonicum strains (CIAT 88, CIAT 89, CIAT 104 and CIAT 105) under aseptic conditions. Forty colonies were isolated from nodules produced by each strain. The progenitor strains, the isolates and four other commercially recommended strains were applied separately to the same cultivar under controlled greenhouse conditions. We observed significant variability in nodulation, shoot dry weight, shoot total N, nodule efficiency (total N mass over nodule mass) and BOX-PCR fingerprinting profiles between variant and progenitor strains. Some variant strains resulted in significantly larger responses in terms of shoot total N, dry weight and nodule efficiency, when compared to their progenitor strain. These results highlight the need for intermittent evaluation of stock bacterial cultures to guarantee effective symbiosis after inoculation. Most importantly, it indicates that it is possible to improve symbiotic effectiveness by screening rhizobial strains for higher N2 fixation capacity within the natural variability that can be found within each progenitor strain. 650 $aNitrogen fixation 650 $aSoybeans 650 $aFixação de nitrogênio 650 $aSoja 700 1 $aKASCHUK, G. 700 1 $aSARIDAKIS, G. P. 700 1 $aCUNHA, M. H. da 773 $tWorld Journal of Microbiology and Biotechnology, Dec. 2011.
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